Composite

Part:BBa_K180012:Design

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-19)

Rock - Primary plasmid (part 2) [tac pR, mCherry and LasI generator]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1825
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1825
    Illegal AgeI site found at 2430
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Regulated expression of LasI (C0178) and mCherry (K180008) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.


Source

See relevant basic biobrick parts (K180000, B0034, K180008, B0015).

References